U-box E3 ubiquitin ligase PUB17 acts in the nucleus to promote specific immune pathways triggered by Phytophthora infestans.
Identifieur interne : 000D42 ( Main/Exploration ); précédent : 000D41; suivant : 000D43U-box E3 ubiquitin ligase PUB17 acts in the nucleus to promote specific immune pathways triggered by Phytophthora infestans.
Auteurs : Qin He [Royaume-Uni] ; Hazel Mclellan [Royaume-Uni] ; Petra C. Boevink [Royaume-Uni] ; Ari Sadanandom [Royaume-Uni] ; Conghua Xie [République populaire de Chine] ; Paul R J. Birch [Royaume-Uni] ; Zhendong Tian [Royaume-Uni]Source :
- Journal of experimental botany [ 1460-2431 ] ; 2015.
Descripteurs français
- KwdFr :
- Apoptose (MeSH), Extinction de l'expression des gènes (MeSH), Feuilles de plante (enzymologie), Feuilles de plante (génétique), Feuilles de plante (immunologie), Immunité des plantes (MeSH), Maladies des plantes (immunologie), Mutation (MeSH), Noyau de la cellule (enzymologie), Phytophthora infestans (physiologie), Protéines végétales (génétique), Protéines végétales (métabolisme), Solanum tuberosum (enzymologie), Solanum tuberosum (génétique), Solanum tuberosum (immunologie), Tabac (enzymologie), Tabac (génétique), Tabac (immunologie), Ubiquitin-protein ligases (génétique), Ubiquitin-protein ligases (métabolisme).
- MESH :
- enzymologie : Feuilles de plante, Noyau de la cellule, Solanum tuberosum, Tabac.
- génétique : Feuilles de plante, Protéines végétales, Solanum tuberosum, Tabac, Ubiquitin-protein ligases.
- immunologie : Feuilles de plante, Maladies des plantes, Solanum tuberosum, Tabac.
- métabolisme : Protéines végétales, Ubiquitin-protein ligases.
- physiologie : Phytophthora infestans.
- Apoptose, Extinction de l'expression des gènes, Immunité des plantes, Mutation.
English descriptors
- KwdEn :
- Apoptosis (MeSH), Cell Nucleus (enzymology), Gene Silencing (MeSH), Mutation (MeSH), Phytophthora infestans (physiology), Plant Diseases (immunology), Plant Immunity (MeSH), Plant Leaves (enzymology), Plant Leaves (genetics), Plant Leaves (immunology), Plant Proteins (genetics), Plant Proteins (metabolism), Solanum tuberosum (enzymology), Solanum tuberosum (genetics), Solanum tuberosum (immunology), Tobacco (enzymology), Tobacco (genetics), Tobacco (immunology), Ubiquitin-Protein Ligases (genetics), Ubiquitin-Protein Ligases (metabolism).
- MESH :
- chemical , genetics : Plant Proteins, Ubiquitin-Protein Ligases.
- enzymology : Cell Nucleus, Plant Leaves, Solanum tuberosum, Tobacco.
- genetics : Plant Leaves, Solanum tuberosum, Tobacco.
- immunology : Plant Diseases, Plant Leaves, Solanum tuberosum, Tobacco.
- chemical , metabolism : Plant Proteins, Ubiquitin-Protein Ligases.
- physiology : Phytophthora infestans.
- Apoptosis, Gene Silencing, Mutation, Plant Immunity.
Abstract
Ubiquitination regulates many processes in plants, including immunity. The E3 ubiquitin ligase PUB17 is a positive regulator of programmed cell death (PCD) triggered by resistance proteins CF4/9 in tomato. Its role in immunity to the potato late blight pathogen, Phytophthora infestans, was investigated here. Silencing StPUB17 in potato by RNAi and NbPUB17 in Nicotiana benthamiana by virus-induced gene silencing (VIGS) each enhanced P. infestans leaf colonization. PAMP-triggered immunity (PTI) transcriptional responses activated by flg22, and CF4/Avr4-mediated PCD were attenuated by silencing PUB17. However, silencing PUB17 did not compromise PCD triggered by P. infestans PAMP INF1, or co-expression of R3a/AVR3a, demonstrating that not all PTI- and PCD-associated responses require PUB17. PUB17 localizes to the plant nucleus and especially in the nucleolus. Transient over-expression of a dominant-negative StPUB17(V314I,V316I) mutant, which retained nucleolar localization, suppressed CF4-mediated cell death and enhanced P. infestans colonization. Exclusion of the StPUB17(V314I,V316I) mutant from the nucleus abolished its dominant-negative activity, demonstrating that StPUB17 functions in the nucleus. PUB17 is a positive regulator of immunity to late blight that acts in the nucleus to promote specific PTI and PCD pathways.
DOI: 10.1093/jxb/erv128
PubMed: 25873665
PubMed Central: PMC4449539
Affiliations:
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Birch</name><affiliation wicri:level="1"><nlm:affiliation>Division of Plant Sciences, University of Dundee, James Hutton Institute (JHI), Errol Road, Invergowrie, Dundee DD2 5DA, UK Dundee Effector Consortium, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK Cell and Molecular Sciences, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK Paul.Birch@hutton.ac.uk tianzhd@mail.hzau.edu.cn.</nlm:affiliation><country wicri:rule="url">Royaume-Uni</country><wicri:regionArea>Division of Plant Sciences, University of Dundee, James Hutton Institute (JHI), Errol Road, Invergowrie, Dundee DD2 5DA, UK Dundee Effector Consortium, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK Cell and Molecular Sciences, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA</wicri:regionArea><wicri:noRegion>Dundee DD2 5DA</wicri:noRegion></affiliation></author><author><name sortKey="Tian, Zhendong" sort="Tian, Zhendong" uniqKey="Tian Z" first="Zhendong" last="Tian">Zhendong Tian</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China and the National Centre for Vegetable Improvement (Central China), Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Paul.Birch@hutton.ac.uk tianzhd@mail.hzau.edu.cn.</nlm:affiliation><country wicri:rule="url">Royaume-Uni</country><wicri:regionArea>Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China and the National Centre for Vegetable Improvement (Central China), Huazhong Agricultural University, Wuhan, Hubei, 430070</wicri:regionArea><wicri:noRegion>430070</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:25873665</idno><idno type="pmid">25873665</idno><idno type="doi">10.1093/jxb/erv128</idno><idno type="pmc">PMC4449539</idno><idno type="wicri:Area/Main/Corpus">000E52</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000E52</idno><idno type="wicri:Area/Main/Curation">000E52</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000E52</idno><idno type="wicri:Area/Main/Exploration">000E52</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">U-box E3 ubiquitin ligase PUB17 acts in the nucleus to promote specific immune pathways triggered by Phytophthora infestans.</title><author><name sortKey="He, Qin" sort="He, Qin" uniqKey="He Q" first="Qin" last="He">Qin He</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China and the National Centre for Vegetable Improvement (Central China), Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Division of Plant Sciences, University of Dundee, James Hutton Institute (JHI), Errol Road, Invergowrie, Dundee DD2 5DA, UK Dundee Effector Consortium, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China and the National Centre for Vegetable Improvement (Central China), Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Division of Plant Sciences, University of Dundee, James Hutton Institute (JHI), Errol Road, Invergowrie, Dundee DD2 5DA, UK Dundee Effector Consortium, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA</wicri:regionArea><wicri:noRegion>Dundee DD2 5DA</wicri:noRegion></affiliation></author><author><name sortKey="Mclellan, Hazel" sort="Mclellan, Hazel" uniqKey="Mclellan H" first="Hazel" last="Mclellan">Hazel Mclellan</name><affiliation wicri:level="1"><nlm:affiliation>Division of Plant Sciences, University of Dundee, James Hutton Institute (JHI), Errol Road, Invergowrie, Dundee DD2 5DA, UK Dundee Effector Consortium, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Division of Plant Sciences, University of Dundee, James Hutton Institute (JHI), Errol Road, Invergowrie, Dundee DD2 5DA, UK Dundee Effector Consortium, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA</wicri:regionArea><wicri:noRegion>Dundee DD2 5DA</wicri:noRegion></affiliation></author><author><name sortKey="Boevink, Petra C" sort="Boevink, Petra C" uniqKey="Boevink P" first="Petra C" last="Boevink">Petra C. Boevink</name><affiliation wicri:level="1"><nlm:affiliation>Dundee Effector Consortium, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK Cell and Molecular Sciences, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Dundee Effector Consortium, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK Cell and Molecular Sciences, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA</wicri:regionArea><wicri:noRegion>Dundee DD2 5DA</wicri:noRegion></affiliation></author><author><name sortKey="Sadanandom, Ari" sort="Sadanandom, Ari" uniqKey="Sadanandom A" first="Ari" last="Sadanandom">Ari Sadanandom</name><affiliation wicri:level="1"><nlm:affiliation>Durham Centre for Crop Improvement Technology School of Biological and Biomedical Sciences, Durham University, Durham DH1 3HP, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Durham Centre for Crop Improvement Technology School of Biological and Biomedical Sciences, Durham University, Durham DH1 3HP</wicri:regionArea><wicri:noRegion>Durham DH1 3HP</wicri:noRegion></affiliation></author><author><name sortKey="Xie, Conghua" sort="Xie, Conghua" uniqKey="Xie C" first="Conghua" last="Xie">Conghua Xie</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China and the National Centre for Vegetable Improvement (Central China), Huazhong Agricultural University, Wuhan, Hubei, 430070, China;</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China and the National Centre for Vegetable Improvement (Central China), Huazhong Agricultural University, Wuhan, Hubei, 430070</wicri:regionArea><wicri:noRegion>430070</wicri:noRegion></affiliation></author><author><name sortKey="Birch, Paul R J" sort="Birch, Paul R J" uniqKey="Birch P" first="Paul R J" last="Birch">Paul R J. Birch</name><affiliation wicri:level="1"><nlm:affiliation>Division of Plant Sciences, University of Dundee, James Hutton Institute (JHI), Errol Road, Invergowrie, Dundee DD2 5DA, UK Dundee Effector Consortium, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK Cell and Molecular Sciences, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK Paul.Birch@hutton.ac.uk tianzhd@mail.hzau.edu.cn.</nlm:affiliation><country wicri:rule="url">Royaume-Uni</country><wicri:regionArea>Division of Plant Sciences, University of Dundee, James Hutton Institute (JHI), Errol Road, Invergowrie, Dundee DD2 5DA, UK Dundee Effector Consortium, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK Cell and Molecular Sciences, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA</wicri:regionArea><wicri:noRegion>Dundee DD2 5DA</wicri:noRegion></affiliation></author><author><name sortKey="Tian, Zhendong" sort="Tian, Zhendong" uniqKey="Tian Z" first="Zhendong" last="Tian">Zhendong Tian</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China and the National Centre for Vegetable Improvement (Central China), Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Paul.Birch@hutton.ac.uk tianzhd@mail.hzau.edu.cn.</nlm:affiliation><country wicri:rule="url">Royaume-Uni</country><wicri:regionArea>Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China and the National Centre for Vegetable Improvement (Central China), Huazhong Agricultural University, Wuhan, Hubei, 430070</wicri:regionArea><wicri:noRegion>430070</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of experimental botany</title><idno type="eISSN">1460-2431</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Apoptosis (MeSH)</term><term>Cell Nucleus (enzymology)</term><term>Gene Silencing (MeSH)</term><term>Mutation (MeSH)</term><term>Phytophthora infestans (physiology)</term><term>Plant Diseases (immunology)</term><term>Plant Immunity (MeSH)</term><term>Plant Leaves (enzymology)</term><term>Plant Leaves (genetics)</term><term>Plant Leaves (immunology)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Solanum tuberosum (enzymology)</term><term>Solanum tuberosum (genetics)</term><term>Solanum tuberosum (immunology)</term><term>Tobacco (enzymology)</term><term>Tobacco (genetics)</term><term>Tobacco (immunology)</term><term>Ubiquitin-Protein Ligases (genetics)</term><term>Ubiquitin-Protein Ligases (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Apoptose (MeSH)</term><term>Extinction de l'expression des gènes (MeSH)</term><term>Feuilles de plante (enzymologie)</term><term>Feuilles de plante (génétique)</term><term>Feuilles de plante (immunologie)</term><term>Immunité des plantes (MeSH)</term><term>Maladies des plantes (immunologie)</term><term>Mutation (MeSH)</term><term>Noyau de la cellule (enzymologie)</term><term>Phytophthora infestans (physiologie)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Solanum tuberosum (enzymologie)</term><term>Solanum tuberosum (génétique)</term><term>Solanum tuberosum (immunologie)</term><term>Tabac (enzymologie)</term><term>Tabac (génétique)</term><term>Tabac (immunologie)</term><term>Ubiquitin-protein ligases (génétique)</term><term>Ubiquitin-protein ligases (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term><term>Ubiquitin-Protein Ligases</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Feuilles de plante</term><term>Noyau de la cellule</term><term>Solanum tuberosum</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Cell Nucleus</term><term>Plant Leaves</term><term>Solanum tuberosum</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plant Leaves</term><term>Solanum tuberosum</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Feuilles de plante</term><term>Protéines végétales</term><term>Solanum tuberosum</term><term>Tabac</term><term>Ubiquitin-protein ligases</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Feuilles de plante</term><term>Maladies des plantes</term><term>Solanum tuberosum</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Plant Diseases</term><term>Plant Leaves</term><term>Solanum tuberosum</term><term>Tobacco</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Plant Proteins</term><term>Ubiquitin-Protein Ligases</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines végétales</term><term>Ubiquitin-protein ligases</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Apoptosis</term><term>Gene Silencing</term><term>Mutation</term><term>Plant Immunity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Apoptose</term><term>Extinction de l'expression des gènes</term><term>Immunité des plantes</term><term>Mutation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Ubiquitination regulates many processes in plants, including immunity. The E3 ubiquitin ligase PUB17 is a positive regulator of programmed cell death (PCD) triggered by resistance proteins CF4/9 in tomato. Its role in immunity to the potato late blight pathogen, Phytophthora infestans, was investigated here. Silencing StPUB17 in potato by RNAi and NbPUB17 in Nicotiana benthamiana by virus-induced gene silencing (VIGS) each enhanced P. infestans leaf colonization. PAMP-triggered immunity (PTI) transcriptional responses activated by flg22, and CF4/Avr4-mediated PCD were attenuated by silencing PUB17. However, silencing PUB17 did not compromise PCD triggered by P. infestans PAMP INF1, or co-expression of R3a/AVR3a, demonstrating that not all PTI- and PCD-associated responses require PUB17. PUB17 localizes to the plant nucleus and especially in the nucleolus. Transient over-expression of a dominant-negative StPUB17(V314I,V316I) mutant, which retained nucleolar localization, suppressed CF4-mediated cell death and enhanced P. infestans colonization. Exclusion of the StPUB17(V314I,V316I) mutant from the nucleus abolished its dominant-negative activity, demonstrating that StPUB17 functions in the nucleus. PUB17 is a positive regulator of immunity to late blight that acts in the nucleus to promote specific PTI and PCD pathways. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25873665</PMID><DateCompleted><Year>2016</Year><Month>03</Month><Day>30</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1460-2431</ISSN><JournalIssue CitedMedium="Internet"><Volume>66</Volume><Issue>11</Issue><PubDate><Year>2015</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Journal of experimental botany</Title><ISOAbbreviation>J Exp Bot</ISOAbbreviation></Journal><ArticleTitle>U-box E3 ubiquitin ligase PUB17 acts in the nucleus to promote specific immune pathways triggered by Phytophthora infestans.</ArticleTitle><Pagination><MedlinePgn>3189-99</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/jxb/erv128</ELocationID><Abstract><AbstractText>Ubiquitination regulates many processes in plants, including immunity. The E3 ubiquitin ligase PUB17 is a positive regulator of programmed cell death (PCD) triggered by resistance proteins CF4/9 in tomato. Its role in immunity to the potato late blight pathogen, Phytophthora infestans, was investigated here. Silencing StPUB17 in potato by RNAi and NbPUB17 in Nicotiana benthamiana by virus-induced gene silencing (VIGS) each enhanced P. infestans leaf colonization. PAMP-triggered immunity (PTI) transcriptional responses activated by flg22, and CF4/Avr4-mediated PCD were attenuated by silencing PUB17. However, silencing PUB17 did not compromise PCD triggered by P. infestans PAMP INF1, or co-expression of R3a/AVR3a, demonstrating that not all PTI- and PCD-associated responses require PUB17. PUB17 localizes to the plant nucleus and especially in the nucleolus. Transient over-expression of a dominant-negative StPUB17(V314I,V316I) mutant, which retained nucleolar localization, suppressed CF4-mediated cell death and enhanced P. infestans colonization. Exclusion of the StPUB17(V314I,V316I) mutant from the nucleus abolished its dominant-negative activity, demonstrating that StPUB17 functions in the nucleus. PUB17 is a positive regulator of immunity to late blight that acts in the nucleus to promote specific PTI and PCD pathways. </AbstractText><CopyrightInformation>© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>He</LastName><ForeName>Qin</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China and the National Centre for Vegetable Improvement (Central China), Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Division of Plant Sciences, University of Dundee, James Hutton Institute (JHI), Errol Road, Invergowrie, Dundee DD2 5DA, UK Dundee Effector Consortium, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>McLellan</LastName><ForeName>Hazel</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Division of Plant Sciences, University of Dundee, James Hutton Institute (JHI), Errol Road, Invergowrie, Dundee DD2 5DA, UK Dundee Effector Consortium, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Boevink</LastName><ForeName>Petra C</ForeName><Initials>PC</Initials><AffiliationInfo><Affiliation>Dundee Effector Consortium, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK Cell and Molecular Sciences, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sadanandom</LastName><ForeName>Ari</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Durham Centre for Crop Improvement Technology School of Biological and Biomedical Sciences, Durham University, Durham DH1 3HP, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xie</LastName><ForeName>Conghua</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China and the National Centre for Vegetable Improvement (Central China), Huazhong Agricultural University, Wuhan, Hubei, 430070, China;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Birch</LastName><ForeName>Paul R J</ForeName><Initials>PR</Initials><AffiliationInfo><Affiliation>Division of Plant Sciences, University of Dundee, James Hutton Institute (JHI), Errol Road, Invergowrie, Dundee DD2 5DA, UK Dundee Effector Consortium, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK Cell and Molecular Sciences, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK Paul.Birch@hutton.ac.uk tianzhd@mail.hzau.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tian</LastName><ForeName>Zhendong</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China and the National Centre for Vegetable Improvement (Central China), Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Paul.Birch@hutton.ac.uk tianzhd@mail.hzau.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>BB/G015244/1</GrantID><Agency>Biotechnology and Biological Sciences Research Council</Agency><Country>United Kingdom</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>04</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Exp Bot</MedlineTA><NlmUniqueID>9882906</NlmUniqueID><ISSNLinking>0022-0957</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.3.2.27</RegistryNumber><NameOfSubstance UI="D044767">Ubiquitin-Protein Ligases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D017209" MajorTopicYN="N">Apoptosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002467" MajorTopicYN="N">Cell Nucleus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020868" MajorTopicYN="N">Gene Silencing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055750" MajorTopicYN="N">Phytophthora infestans</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D057865" MajorTopicYN="Y">Plant Immunity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011198" MajorTopicYN="N">Solanum tuberosum</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D044767" MajorTopicYN="N">Ubiquitin-Protein Ligases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Disease resistance</Keyword><Keyword MajorTopicYN="N">effector-triggered 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Birch</name><name sortKey="Boevink, Petra C" sort="Boevink, Petra C" uniqKey="Boevink P" first="Petra C" last="Boevink">Petra C. Boevink</name><name sortKey="Mclellan, Hazel" sort="Mclellan, Hazel" uniqKey="Mclellan H" first="Hazel" last="Mclellan">Hazel Mclellan</name><name sortKey="Sadanandom, Ari" sort="Sadanandom, Ari" uniqKey="Sadanandom A" first="Ari" last="Sadanandom">Ari Sadanandom</name><name sortKey="Tian, Zhendong" sort="Tian, Zhendong" uniqKey="Tian Z" first="Zhendong" last="Tian">Zhendong Tian</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Xie, Conghua" sort="Xie, Conghua" uniqKey="Xie C" first="Conghua" last="Xie">Conghua Xie</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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